Russian and Belarusian players will not be allowed to compete at Wimbledon this year because of the invasion of Ukraine.
Men's world number two Daniil Medvedev of Russia and women's world number four Aryna Sabalenka of Belarus are the highest-ranked players to be affected.
The All England Lawn Tennis Club (AELTC) said it had a responsibility to "limit Russia's global influence through the strongest means possible".
READ ALSO: US Open: Daniil Medvedev stuns Novak Djokovic to win maiden Grand Slam title
Wimbledon runs from June 27 to July 10.
"In the circumstances of such unjustified and unprecedented military aggression, it would be unacceptable for the Russian regime to derive any benefits from the involvement of Russian or Belarusian players," a statement from the AELTC read.
"It is therefore our intention, with deep regret, to decline entries from Russian and Belarusian players to Wimbledon."
Sabalenka reached the semi-finals of last year's tournament, while Medvedev, who has been announced as one of the star draws at the grass-court warm-up event at 's-Hertogenbosch in the Netherlands today, reached the fourth round.
Russian world number 15 Anastasia Pavlyuchenkova - who called for the war to stop earlier this year - and 18th-ranked Victoria Azarenka of Belarus will also miss out.
Russia's Andrey Rublev is eighth in the men's standings, with compatriot Karen Khachanov 26th.
The Lawn Tennis Association has also banned Russian or Belarusian players from playing at any of the UK grass court tournaments.
They will all still be able to compete at the French Open, which begins in May.
Players from both countries have been allowed to compete on the tennis tour but not under their national flags.
Kremlin spokesperson Dmitry Peskov criticised the ban.
"Given that Russia is a strong tennis country and our athletes possess top lines of the world rating, the tournament itself would suffer because of this ban," he said.
"It is unacceptable to make the athletes once again hostages of certain political prejudice, intrigues and hostile actions towards our country."
The AELTC, which organises Wimbledon, consulted the government in April about whether to allow players to compete.
"We recognise that this is hard on the individuals affected, and it is with sadness that they will suffer for the actions of the leaders of the Russian regime," AELTC chairman Ian Hewitt said.
"Given the importance of not allowing sport to be used to promote the Russian regime and our broader concerns for public and player (including family) safety, we do not believe it is viable to proceed on any other basis at The Championships."
A statement from the LTA said it is "important to do all it can to support Ukraine at this time".
"LTA believes that tennis must join many other areas of sport and public life in sending a clear signal to the Russian and Belarusian states that their actions are the subject of international condemnation," a statement read.
"The continuing participation of Russian and Belarusian nationals at events risks providing a boost to these regimes when there is an unprecedented international effort to isolate them and sanction their actions."
READ ALSO: Rafael Nadal beats Daniil Medvedev in epic final to win Australian Open
Sports minister Nigel Huddleston previously said "nobody flying the flag for Russia should be allowed" to play at Wimbledon.
However, WTA head Steve Simon told BBC Sport in March that he did not believe players from the two countries should be banned from tournaments.
Russia was previously banned from defending its Davis Cup and Billie Jean King Cup team titles after the country's invasion of Ukraine - a military operation supported by Belarus.
The Women's Tennis Association (WTA) and the men's association (ATP) have suspended their combined event scheduled to take place in Moscow in October.
The International Tennis Federation (ITF) has also cancelled its events in the country.
'There comes a time when silence is betrayal'
Ukrainian world number 25 Elina Svitolina released a lengthy statement on Wednesday, calling on tennis' governing bodies to take a harsher stance against Russian and Belarusian athletes.
Svitolina has taken a break from the sport to deal with a back problem and the emotional impact the invasion has had on her.
She posted on social media urging the ATP, WTA and ITF to ask Russian and Belarusian players if they supported the war, the military activities in Ukraine or the regimes of Russian and Belarusian presidents Vladimir Putin and Alexander Lukashenko.
"If applicable, we demand to exclude and ban any Russian and Belarusian athletes from competing in any events," she wrote.
"In times of crisis, silence means agreeing with what is happening.
"There comes a time when silence is betrayal, and that time is now."
‼️💙💛🇺🇦✊🏼 pic.twitter.com/UK04g8OOh9
— Elina Monfils (@ElinaSvitolina) April 20, 2022
Olga Savchuk, who captained Ukraine in last week's Billie Jean King Cup tie against the USA, said Russian players should be banned from competing.
"It cannot just be a sanction against 90% of the Russian people and 10% not," Savchuk told the New York Times.
"It has to be even and I think it is collective guilt."
Ukrainian former world number 13 Alexandr Dolgopolov thanked Wimbledon for "stepping up and showing the world an example".
"I believe Russia should be isolated in all possible ways, and the people of Russia have to solve this problem," he told BBC Sport.
BBC